Ointment Formulation from Collagen Extract of Tilapia Fish Skin (Oreochromis niloticus) for Healing Burns in Mus musculus

Authors

  • Romy Triadi Nugroho Chemistry, Matematics and Natural Sciences, Faculty of Science and Technology, Universitas Jambi
  • Gilang Saputra Chemistry, Matematics and Natural Sciences, Faculty of Science and Technology, Universitas Jambi
  • Annisa Nurul Aini Chemistry, Matematics and Natural Sciences, Faculty of Science and Technology, Universitas Jambi
  • Aisha Andini Indira Dewi Chemistry, Matematics and Natural Sciences, Faculty of Science and Technology, Universitas Jambi
  • Indra Lasmana Tarigan Faculty of Science and Technology, Universitas Jambi

DOI:

https://doi.org/10.21776/ub.pji.2022.008.01.2

Keywords:

Collagen Extract, Ointment Formulation, Burns

Abstract

Introduction: Burns are injuries that caused by contact with a heat source. The natural alternative burn treatment can use hydrolyzed collagen derived from aquatic ecosystems, one of them is fish.

Objectives: The aim of this research was to find the optimum formulation of tilapia fish collagen extract ointment.

Methods: Fish skin that has been separated was prepared using the solution of NaOH 0,1 N and Butyl Alcohol 10%.Then the fish skin was extracted using a 0.5 M acetic acid solution and precipitated with a 0.9 M NaCl solution. Then, dialysis was performed using a plastic membrane (14 KDa) in a 0.1 M acetic acid solution and distilled water to obtain a wet collagen extract which was then in the FreezeDryer to reduce the water content.

Results: The collagen extract obtained was used for ointment formulations with different concentrations of collagen extract, that is 5%, 10%, and 15% and 2 additional miana plant extracts were used with collagen extract 5+5% and 10+10%. The results of the formulation were tested on burns with a diameter of 0.715 cm in mice.

Conclusion: From the results of the effectiveness test, the formulation with 15% collagen extract showed effective results. Indicated by the rate of wound healing in mice observations

Author Biographies

Romy Triadi Nugroho, Chemistry, Matematics and Natural Sciences, Faculty of Science and Technology, Universitas Jambi

Chemistry, Matematics and Natural Sciences, Faculty of Science and Technology, Universitas Jambi

Gilang Saputra, Chemistry, Matematics and Natural Sciences, Faculty of Science and Technology, Universitas Jambi

Chemistry, Matematics and Natural Sciences, Faculty of Science and Technology, Universitas Jambi

Annisa Nurul Aini, Chemistry, Matematics and Natural Sciences, Faculty of Science and Technology, Universitas Jambi

Chemistry, Matematics and Natural Sciences, Faculty of Science and Technology, Universitas Jambi

Aisha Andini Indira Dewi, Chemistry, Matematics and Natural Sciences, Faculty of Science and Technology, Universitas Jambi

Chemistry, Matematics and Natural Sciences, Faculty of Science and Technology, Universitas Jambi

Indra Lasmana Tarigan, Faculty of Science and Technology, Universitas Jambi

Chemistry, Matematics and Natural Sciences, Faculty of Science and Technology, Universitas Jambi 

Center for Halal Studies, LPPM, Universitas Jambi

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Published

2022-12-30

Issue

Vol. 8 No. 1 (2022)

Section

Articles

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