The Sensitivity of Amphotericin B, Fluconazole, and Flusitosin to Fungal Pathogens Isolated from Wounds of Diabetic Ulcer Patients

Authors

  • Rafika Sari Tanjungpura University

DOI:

https://doi.org/10.21776/ub.pji.2022.007.02.01

Keywords:

Anti-fungus, Diabetic Ulcer Wound, Sensitivity

Abstract

A diabetic ulcer is a wound caused by infection, ulceration or destruction in people with diabetes mellitus. Mostly, diabetic ulcer found in foot. The purpose of this study was to identify pathogenic fungi in diabetic ulcers and their sensitivity to fungal drugs. The morphological determination of fungal isolates was stained with Lactophenol Cotton Blue. Determination of sensitivity using diffusion discs Kirby Bauer which are classified based on Clinical Laboratory Standard Institute (CLSI) with Amphotericin B, Fluconazole, and Flusitosin. Based on the results of the study found 8 types of pathogenic fungi namely Candida albicans (38.78%), Candida guilliermondii (4.08%), Candida kefyr 10.20%), Candida parapsilosis (10.20%), Fusarium solani (14.29) %), Microsporum audouinii (14.29%), Penicillium sp(2.04%), Trichophyton mentagrophytes (4.08%), and Trichophyton equinum (2.04%). The fungus was identified using Gideon software and the book Description of Medical Fungi. The results of the antifungal sensitivity test showed the results were sensitive to fluconazole. Amphotericin B is intermediate to Candida albicans, Candida guilliermondii, Penicillium sp. and Trichophyton sp. and is resistant to Candida kefyr and Candida parapsilosis. Flusitosin is only an intermediate against Penicillium sp. while other fungal species are immune. The conclusion of this study. The results of diabetic ulcer swabs III and IV indicate the presence of fungal colonies with species of 9 species namely Candida albicans, Candida guilliermondii, Candida kefyr, Candida Parapsilosis, Fusarium solani, Microsporum Audouinii, Penicillium sp., Trichophyton mentagrophytes, and Trichophyton equinum. Antifungal sensitivity tests on all pathogenic fungi showed sensitivity to fluconazole. Amphotericin Bintermediatrics against Candida albicans, Candida guilliermondii, Penicillium sp. and Trichophyton sp. while resistant to Candida kefyrand Candida parapsilosis. Flusitosin is only an intermediate against Penicillium sp. while other fungal species are resistant.

Author Biography

Rafika Sari, Tanjungpura University

Department of Pharmacy, Faculty of Medicine

References

Roza RL, Rudy A, Zulkarnain E. Faktor Risiko Terjadinya Ulkus Diabetikum pada Pasien Diabetes Mellitus yang Dirawat Jalan dan Inap di RSUP Dr. M. Djamil dan RSI Ibnu Sina Padang. J Kesehat Andalas 2015;4(1):243.

Nurmansyah AS, Rochmawati E, Primanda Y. Dukungan Keluarga pada Pasien Luka Kaki Diabetes di Klinik Kitamura Pontianak: Studi Fenomenologi. 2018;

Santosh Y, Ramanath K, Naveen M. Fungal Infections In Diabetes Mellitus: An Overview. Int J Pharm Sci Rev Res 2011;7(2):222.

Suarez MG. Identification and Antifungal Susceptibility of Penicillium-Like Fungi From Clinical Samples in The United States. J Clin Microbiol Am Soc Microbiol 2016;54(8).

Siswandono, Soekardjo B. Kimia Medisinal. 2nd ed. Surabaya: Airlangga University Press; 2000.

Hendrawati TY, Utomo S. Optimasi Suhu dan Waktu Sterilisasi pada Kualitas Susu Segar di Kabupaten Boyolali. J Teknol Univ Muhammadiyah Jakarta 2017;9(2).

Fauziyyah NA, Putri DAS. Isolasi Jamur dari Batuan Penutup Drainase Pada Sisi Selatan Lantai II Bidang H Candi Borobudur. J Konserv Cagar Budaya Borobudur 2016;10(2):40–4.

Deorukhkar SC, Shahriar R. Identification of Candida Species: Conventional Methods in the Era of Molecular Diagnosis. Ann Microbiol Immunol (Review Artic 2018;1(1):1–4.

Kidd S, Catriona H, Helen A, David E. Descriptions Of Medical Fungi. Third Edit. Australia: National Mycology Reference Centre; 2016.

Wayne P. Method for Antifungal Disk Diffusion Susceptibility Testing of Yeast Approved Guideline CLSI document M44-A2. Second Edi. Clinical and Laboratory Standards Institute; 2009.

Cockerill FR, Matthew AW, Jeff A, Michael ND, George ME, Mary JF. Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard. CLSI document M02-A11. Eleventh E. Clinical and Laboratory Standards Institute; 2012.

Dewi A. Hubungan Aspek-aspek Perawatan Kaki Diabetes dengan Kejadian Ulkus Kaki Diabetes pada Pasien Diabetes Mellitus. Mutiara Med 2007;7(1):13–21.

Waspadji S. Penatalaksanaan Diabetes Melitus Terpadu. Jakarta: Balai Penerbit Fakultas Kedokteran, Universitas Indonesia; 2005.

Jawetz E, JL M, EA A. Mikrobiologi Kedokteran. Jakarta: EGC; 1996.

Sarenje K. Distribution and Antifungal Susceptibility of Candida Species Isolated from Clinical Specimens at the University Teaching Hospital, Lusaka, Zambia. IOSR J Pharm 2017;7(2).

Pelczar J, Chan ECS. Dasar-Dasar Mikrobiologi. Jilid 2. Jakarta: UI Press; 2005.

Verma S, Heffernan M. Superficial fungal infection: dermatophytosis, onychomycosis, tinea nigra, piedra. Seventh Ed. New York: McGraw Hill; 2010

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Published

2022-06-30

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